4 posts
A concrete, runnable pipeline that takes FASTQ files from whole-genome and RNA sequencing through alignment, variant calling, and expression quantification, wrapped in Nextflow so it reruns cleanly.
A step-by-step guide to turning raw FASTQ files from whole-genome and RNA sequencing into aligned, called, annotated, and validated results on your own hardware, with the specific tools, flags, and quality thresholds we use.
A practical pipeline for taking personal bulk RNA-seq FASTQs through quality control, quantification, normalization, and interpretation, with the parameters and failure modes that matter when n=1.
A 2026 guide to the RNA-seq workflow. Covers experimental design, data analysis and building reproducible pipelines.